09-543: Mass Spectrometry: Fundamentals, Instrumentation and Techniques

Department:

Chemistry

Units:

6.0

Related:

http://www.chem.cmu.edu/

This course is intended for students interested in understanding fundamentals, instrumentation and techniques used in mass spectrometry(MS). The operating principles of various ion sources (EI, CI, ESI and MALDI), mass analyzers (magnetic, quadrupole, time-of-flight, ion traps) and detectors are covered. Applications are focused in the areas of small molecule, proteomics and polymer characterization. Protein identification, peptide sequencing, polymer polydispersity and end group determination are covered. Hyphenated techniques such as GC/MS, LC/MS, and LC/QTOF MS are covered. Various MS scan modes (i.e., SIM, SRM, MS/MS) and basic spectrum interpretation are covered. Students are exposed to QET and RRKM theory and select gas phase reactions. The lecture is supplemented with the use of the "Virtual Mass Spectrometry Laboratory" Internet tool, remote control of mass spectrometers and laboratory. 3 hrs. lec., 1.5 hrs. lab.

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